Human Anti-HIV-1 Env Recombinant Antibody (clone PGDM1400)
CAT#: PABL-151
Recombinant Human Antibody (PGDM1400) is capable of binding to HIV-1 env, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 env mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 env mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Tested Data
SDS-PAGE
Figure 1 Human Anti-HIV-1 Env Recombinant Antibody (PABL-151) in SDS-PAGE
SDS-PAGE analysis of PABL-151 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, the different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABL-151 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Human Anti-HIV-1 Env Recombinant Antibody (PABL-151) in SEC-HPLC
The purity of PABL-151 was 98.9773% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Human Anti-HIV-1 Env Recombinant Antibody (PABL-151) in WB
Western blot analysis of PABL-151 was performed by loading recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein, (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-151 and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 4 Human Anti-HIV-1 Env Recombinant Antibody (PABL-151) in ELISA
ELISA analysis of PABL-151 was performed by coating with recombinant HIV-1 [HIV-1/Clade B/C (CN54)] gp120 protein, (His tag). Then blocked with BSA and incubated with PABL-151. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Species Reactivity
- HIV-1
- Clone
- PGDM1400
- Applications
- Neut, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Neut and FuncS. For details, refer to published data.
Target
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(Creative Biolabs Cat# PABL-151, RRID: AB_3111629)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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